Date of Award
12-4-2006
Degree Type
Thesis
Degree Name
Master of Science (MS)
Department
Chemistry
First Advisor
Dr. Gabor Patonay - Chair
Second Advisor
Dr. Lucjan Strekowski
Third Advisor
Dr. Jerry C. Smith
Abstract
A rapid and accurate quantitative method was developed and validated for the analysis of four urinary organic acids with nitrogen containing functional groups, formiminoglutamic acid (FIGLU), pyroglutamic acid (PYRGLU), 5-hydroxyindoleacetic acid (5-HIAA), and 2-methylhippuric acid (2-METHIP) by liquid chromatography tandem mass spectrometry (LC/MS/MS). The chromatography was developed using a weak anion-exchange amino column that provided mixed-mode retention of the analytes. The elution gradient relied on changes in mobile phase pH over a concave gradient, without the use of counter-ions or concentrated salt buffers. A simple sample preparation was used, only requiring the dilution of urine prior to instrumental analysis. The method was validated based on linearity (r2 ¡Ý 0.995), accuracy (85¨C115%), precision (C.V. < 12%), sample preparation stability (¡Ü 5%, 72h), and established patient ranges. The method was found to be both efficient and accurate for the analysis of urinary zwitterionic organic acids.
Recommended Citation
Bishop, Michael Jason, "The Application of Weak-Anion Exchange Chromatography for the Analysis of Organic Zwitterions Using LC/MS/MS" (2006). Chemistry Theses. Paper 5.
http://digitalarchive.gsu.edu/chemistry_theses/5